Mark Skinner, director of European retail at Henderson, has left the business as part of the latest re-shuffle at the asset manager.
Skinner, who joined the group in 2009 following its takeover of New Star, left the business at the start of January, the group said.
Skinner is the latest well-known figure to exit the business, following the departure of Arno Kitts - co-head of global distribution - last year.
Separately, the group said Stephen Peak, manager of the underperforming £439m Henderson UK Alpha Fund, is handing over his fund to Neil Hermon and James Ross at the start of February.
The fund is one of the poorest performers in the IMA UK All Companies sector over the last year, delivering a return of just 5.2%, compared to a sector average return of 16%, more than three times higher.
The group's global financials team, led by Simon Peters, has also departed the firm, with its £54m Global Financials fund moving to Matthew Beesley's team, which currently runs global equities at the firm. The company has also opted to close its global financials hedge fund, as part of the latest overhaul.
Henderson said Peak will now concentrate on the group's US mutual funds business, as well as having responsibility for the global equity investment unit.
He remains lead portfolio manager of the US mutual funds - the European Focus fund and the International Opportunities fund.
Graham Kitchen, head of equities, said: "Not only is it vital that we provide the best investment teams to all our clients' funds throughout Henderson but also that the managers have the capacity to fully focus on each of our clients.
"James has really proved himself since joining Henderson on the graduate scheme in 2007 and is ‘one to watch' in the coming years. Bringing Neil and James together on the fund ensures the fund will be in extremely capable hands."
Meanwhile James Senior, formerly head of marketing at Ignis, has joined Henderson as a marketing consultant. He will report to Allyson Foster, head of UK retail marketing.
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